Nuclear Magnetic Resonance Division
Description
NMR is a spectroscopic technique based on the magnetic properties of the nuclei of certain isotopes of elements such as 1H, 13C, 15N, 31P, 11B, 19F, etc. When the samples are subjected to excitation with radio waves, a complex signal is produced which, when properly processed, generates an NMR spectrum. The position and intensity of the signals, together with a detailed analysis of their fine structure, allows information to be obtained on the type of atoms present in the sample as well as their connection and spatial distribution, leading to a detailed study of the molecular structure.
Lines of research
• Confirmation of structures.
• Identification of compounds.
• Studies of mixture composition.
• Determination of purity, quantification.
• Molecular mobility: determination of conformations in solution.
• Monitoring of processes, reactions, kinetics.
• High and low temperature studies.
• Determination of enantiomeric excess.
Type of test
One-dimensional spectra:
• One-dimensional experiments of 1H, 13C, 19F, 31P and others.
• Selective PRESAT experiments, Selective-1D, TOCSY1D, NOESY1D, ROESY1D.
• Experiments with 13C detection: APT, DEPT.
Two-dimensional experiments:
• Homonuclear correlation: COSY, g-COSY, g- DQCOSY, TOCSY, NOESY and ROESY.
• Heteronuclear correlation (with inverse detection): HMQC, g-HMQC, HSQC, g-HSQC, HMBC, g-HMBC, HMQCTOCSY, g-HMQCTOCSY, HS¬QCTOCSY, g-HSQCTOCSY.
Diffusion experiments: DOSY
Infrastructures
• Agilent 500 MHz NMR spectrometer, multinuclear automatic probe and HRMAS probe
• Agilent 600 MHz NMR spectrometer, inverse triple probe and cryoprobe (inverse).
Contact
Facultad de Ciencias
11510, Campus de Puerto Real, Cádiz
ana.simonet@uca.es (Responsable división)
juanmiguel.duarte@uca.es ( Técnico división)
https://sccyt.uca.es
